Abstract
Detailed studies of chromosome rearrangements within solid tumors require karyotype
analysis after cell culturing. However, different cell subpopulations with various
growth capacities within one tumor may introduce biases in karyotype analysis, known
as the in vitro selection. In our laboratory, 22% of karyotypes from breast cancers
established after short-term culture were normal. Using interphase fluorescence in
situ hybridization (FISH) for the determination of chromosome 1 arm imbalances and
flow cytometry measurements of ploidy, we demonstrated that at least 2/3 of these
tumors were mainly composed of aneuploid cell populations. Thus, the incidence of
normal or balanced karyotypes among breast cancers is probably below 7%. This is the
first direct proof for the existence of an in vitro selection within breast cancer
cultures, suggesting cautious interpretation of cytogenetic data.
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References
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Article info
Publication history
Accepted:
March 11,
1999
Received:
December 10,
1998
Identification
Copyright
© 1999 Elsevier Science Inc. Published by Elsevier Inc. All rights reserved.
