Abstract
We attempted to define the relation between DNA replication errors (RERs) in endometrial
carcinomas and the precancerous lesion complex atypical endometrial hyperplasia (ATH)
and clinicopathological characteristics. Tissue samples from 93 patients with endometrial
carcinoma diagnosed as endometrioid adenocarcinoma and 26 patients with ATH (including
21 in whom endometrial carcinoma also was found) were prepared as formalin-fixed,
paraffin-embedded sections. The samples were examined for the presence of RERs by
the polymerase chain reaction with the use of five microsatellite markers. RERs were
observed at ≥1 loci in 32 endometrial carcinoma patients (34%); all 26 ATH patients were RER negative.
RERs were observed in 25% of stage I and stage II cancer patients (16/64) and in 55%
of stage III and stage IV cancer patients (16/29) (P = 0.009), as well as in 63% (10/16)
of cancer patients with and in 27% (20/75) of patients without lymph-node metastases
(P = 0.013). The incidence of RERs was not related to patient age, histological tumor
grade, or prognosis. These results suggest that RER may be involved in the advanced
rather than the early stages of endometrioid adenocarcinoma. There appears to be little
association between RER and ATH.
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Article info
Publication history
Accepted:
March 29,
1999
Received:
November 3,
1998
Identification
Copyright
© 1999 Elsevier Science Inc. Published by Elsevier Inc. All rights reserved.
