Abstract
Thirteen chronic myeloid leukemia (CML) patients, 10 with variant Philadelphia (Ph)
translocations and 3 Ph negative cases, were analyzed by fluorescence in situ hybridization
(FISH) with the use of BCR and ABL cosmid probes and a chromosome 22 painting probe.
In the variant Ph translocations, the BCR-ABL fusion gene was located on the Ph chromosome; in 1 CML Ph-negative patient, the BCR-ABL fusion gene was located on the Ph chromosome; and, in 2 patients, it was located
on chromosome 9. The chromosome 22 painting probe was detected on the third-party
chromosome of the variant translocation, and in none of the variant translocations
was there any detectable signal on chromosome 9. In CML patients with clonal evolution
of a simple Ph, a signal of the chromosome 22 painting probe was detected on the der(9)
of the Ph translocation. It was concluded that the variant Ph translocations evolved
simultaneously in a three-way rearrangement. The clinical parameters of the 13 patients
were similar to those of a large group of CML patients with a simple Ph translocation.
It is suggested that, to determine the prognosis of CML patients with a complex karyotype,
FISH analysis with a chromosome 22 painting probe be performed.
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Article info
Publication history
Accepted:
March 30,
1999
Received:
November 17,
1998
Identification
Copyright
© 1999 Elsevier Science Inc. Published by Elsevier Inc. All rights reserved.
