Circ-SLC7A5, a potential prognostic circulating biomarker for detection of ESCC

  • Author Footnotes
    1 They contributed equally to this work.
    Qian Wang
    Footnotes
    1 They contributed equally to this work.
    Affiliations
    Department of Oncology Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
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  • Author Footnotes
    1 They contributed equally to this work.
    Han Liu
    Footnotes
    1 They contributed equally to this work.
    Affiliations
    Department of Oncology Surgery, Taikang Xianlin Drum Tower Hospital, Nanjing University, Nanjing, China
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  • Author Footnotes
    1 They contributed equally to this work.
    Zihao Liu
    Footnotes
    1 They contributed equally to this work.
    Affiliations
    Department of Oncology, Suqian Hospial Affiliated to Xuzhou Medical University, Suqian, China
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  • Lili Yang
    Affiliations
    Department of Oncology Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
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  • Jian Zhou
    Affiliations
    Department of Oncology Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
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  • Xiufeng Cao
    Correspondence
    Corresponding authors at: Department of Oncology Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
    Affiliations
    Department of Oncology Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, China

    Department of Oncology Surgery, Taikang Xianlin Drum Tower Hospital, Nanjing University, Nanjing, China
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  • Handong Sun
    Correspondence
    Corresponding authors at: Department of Oncology Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
    Affiliations
    Department of Oncology Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
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  • Author Footnotes
    1 They contributed equally to this work.

      Highlights

      • Circular RNAs (circRNAs) have permanently altered our perspectives toward cancer recently, especially in carcinogenesis and cancer progression.
      • However, the roles of circRNAs in esophageal squamous cell carcinoma (ESCC) remain largely unknown.
      • In our study, 10 plasma samples from the patients at different TNM stages and 5 normal controls were used to detect circRNAs expression profiles in ESCC.
      • ESCC tissues can secret circRNAs into plasma and patients with high plasma circ-SLC7A5 are associated with high TNM stage, tending to have shorter overall survival than those with low levels.
      • In addition, the biological characteristics of circ-SLC7A5 including location, miRNAs binding, m6A modification were found to be related to ESCC process.
      • The study reveals a novel prognosis biomarker of circ-SLC7A5, providing a preliminary landscape of circRNA expression for detection of ESCC.

      Abstract

      Circular RNAs (circRNAs), resulting from the non-canonical splicing of linear pre-mRNAs, have permanently altered our perspectives toward cancer recently, especially in carcinogenesis and cancer progression. However, the roles of circRNAs in esophageal squamous cell carcinoma (ESCC) remain largely unknown. In the current study, circRNAs expression profiles are screened in ESCC, using plasma samples from 10 ESCC patients, including different TNM stages and 5 normal controls. Characteristics of circRNAs including length, types, and the possibility of binding to proteins are analyzed. Candidate tumor-related circRNAs are then quantitated in ESCC tissues, plasmas and cell lines. ESCC tissues can secret circRNAs into plasma and patients with high plasma circ-SLC7A5 are associated with high TNM stage, tending to have shorter overall survival than those with high levels. In addition, the biological characteristics of circ-SLC7A5 including location, miRNAs binding, m6A modification were analyzed. Our study reveals a novel prognosis biomarker of circ-SLC7A5, providing a preliminary landscape of circRNA expression for detection of ESCC.

      Keywords

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