Highlights
- •Establishment of a rapid FISH method with a turn around time of less than one hour.
- •FISH hybridization time was reduced to 10 min using Vysis IntelliFISH buffer.
- •Allowed the combined same day reporting of morphology and FISH results.
- •There was no detrimental effect on the quality or accuracy of the FISH results.
- •Enabled clinicians to have rapid access to targeted therapies.
Abstract
This study evaluated a rapid fluorescence in-situ hybridization (FISH) method with
a novel 10 min hybridization time to identify the presence of recurrent gene re-arrangements
in patients with Haematological malignancies (HMs).
A method comparison experimental approach was used to compare this rapid method to
the standard method. Hybridization results using the rapid method were comparable
to standard methods in terms of result, signal strength and hybridization quality.
This rapid FISH turn around time (TAT) was 1 h and enabled same day reporting of genetic
and morphology results for a prompt diagnosis and timely access to targeted therapies.
Keywords
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Article Info
Publication History
Published online: April 08, 2022
Accepted:
March 9,
2022
Received in revised form:
February 18,
2022
Received:
December 2,
2021
Identification
Copyright
Crown Copyright © 2022 Published by Elsevier Inc. All rights reserved.
