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Rapid FISH results within one hour

  • Catherine Wren
    Correspondence
    Corresponding author.
    Affiliations
    Institute of Clinical Pathology and Medical Research, New South Wales Health Pathology, Westmead Hospital, Westmead, NSW 2145, Australia
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  • Elizabeth Tegg
    Affiliations
    Institute of Clinical Pathology and Medical Research, New South Wales Health Pathology, Westmead Hospital, Westmead, NSW 2145, Australia

    University of Sydney, Westmead Medical School, Westmead, NSW 2145, Australia
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      Highlights

      • Establishment of a rapid FISH method with a turn around time of less than one hour.
      • FISH hybridization time was reduced to 10 min using Vysis IntelliFISH buffer.
      • Allowed the combined same day reporting of morphology and FISH results.
      • There was no detrimental effect on the quality or accuracy of the FISH results.
      • Enabled clinicians to have rapid access to targeted therapies.

      Abstract

      This study evaluated a rapid fluorescence in-situ hybridization (FISH) method with a novel 10 min hybridization time to identify the presence of recurrent gene re-arrangements in patients with Haematological malignancies (HMs).
      A method comparison experimental approach was used to compare this rapid method to the standard method. Hybridization results using the rapid method were comparable to standard methods in terms of result, signal strength and hybridization quality.
      This rapid FISH turn around time (TAT) was 1 h and enabled same day reporting of genetic and morphology results for a prompt diagnosis and timely access to targeted therapies.

      Keywords

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